The Human CD5L (CD5 Antigen-like) ELISA Kit is specifically designed to accurately measure CD5L levels in human samples including serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.CD5L, also known as CD5 antigen-like, is a protein that plays a critical role in regulating immune responses and inflammation.
It is implicated in various diseases including autoimmune disorders, cancer, and infectious diseases, making it a valuable biomarker for studying these conditions and developing potential treatments.This ELISA kit offers a convenient and efficient method for quantifying CD5L levels in human samples, providing researchers with valuable insights into the role of CD5L in disease pathogenesis and potential therapeutic interventions.
Product Name:
Human CD5L (CD5 Antigen-like) ELISA Kit
SKU:
HUES03547
Target:
Human CD5L (CD5 Antigen-like)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CD5L. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CD5L and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CD5L, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CD5L. You can calculate the concentration of Human CD5L in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-106
89-103
95-110
Average (%)
99
96
103
1:4
Range (%)
88-100
80-93
88-101
Average (%)
94
87
94
1:8
Range (%)
91-102
83-95
81-96
Average (%)
96
88
88
1:16
Range (%)
91-105
82-91
88-101
Average (%)
96
86
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-103
97
EDTA plasma (n=5)
86-102
93
Cell culture media (n=5)
89-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.56
7.28
24.3
2.65
6.84
23.27
Standard deviation
0.16
0.38
0.85
0.14
0.33
0.83
C V (%)
6.25
5.22
3.5
5.28
4.82
3.57
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CD5L concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CD5L in samples. No significant cross-reactivity or interference between Human CD5L and analogues was observed.
