Human CDKN2A (Cyclin Dependent Kinase Inhibitor 2A) ELISA Kit
Based on the information provided at the URL, the Human CDKN2A (Cyclin-Dependent Kinase Inhibitor 2A) ELISA Kit is specifically designed for the accurate detection of CDKN2A levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications. CDKN2A is a critical protein involved in cell cycle regulation, acting as a tumor suppressor to maintain genomic stability and prevent abnormal cell growth.
Dysregulation of CDKN2A is associated with various malignancies, including melanoma and pancreatic cancer, highlighting its importance as a biomarker in cancer research and potential therapeutic targeting. Researchers can rely on the Human CDKN2A ELISA Kit to effectively measure CDKN2A levels in human samples, enabling a deeper understanding of its role in cancer biology and providing valuable insights for future diagnostic and treatment strategies.
Product Name:
Human CDKN2A (Cyclin Dependent Kinase Inhibitor 2A) ELISA Kit
SKU:
HUES03202
Target:
Human CDKN2A (Cyclin Dependent Kinase Inhibitor 2A)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CDKN2A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CDKN2A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CDKN2A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CDKN2A. You can calculate the concentration of Human CDKN2A in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-112
84-97
86-98
Average (%)
103
91
92
1:4
Range (%)
94-106
80-89
83-95
Average (%)
99
85
90
1:8
Range (%)
90-102
81-96
87-98
Average (%)
96
87
93
1:16
Range (%)
90-103
79-93
86-99
Average (%)
96
86
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
99
EDTA plasma (n=5)
95-107
101
Cell culture media (n=5)
86-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.08
6.28
16.73
1.92
6.51
15.76
Standard deviation
0.11
0.37
0.8
0.11
0.37
0.68
C V (%)
5.29
5.89
4.78
5.73
5.68
4.31
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CDKN2A concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CDKN2A in samples. No significant cross-reactivity or interference between Human CDKN2A and analogues was observed.
