The CFD ELISA Kit is meticulously designed to facilitate the precise and quantitative detection of Complement Factor D (CFD) levels in human biological samples. CFD, a pivotal component of the alternative pathway of the complement system, plays a crucial role in immune responses, particularly in the activation of the immune cascade. Monitoring CFD levels is essential for unraveling its involvement in immune regulation, host defense, and inflammatory processes.
Accurate measurement of CFD provides valuable insights into its role in various diseases, immune disorders, and infections, aiding in the development of targeted therapeutic interventions. Our CFD ELISA Kit offers outstanding sensitivity and specificity, ensuring dependable and reproducible results in your research or clinical endeavors. With stringent quality control measures during manufacturing, this kit ensures robust performance and user-friendly operation for reliable quantification of CFD. Count on our CFD ELISA Kit for precise and trustworthy determination of this essential biomarker in your investigative studies.
Product Name:
Human CFD (Complement Factor D) ELISA Kit
SKU:
AEES00046
Target:
Human CFD (Complement Factor D)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CFD. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CFD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CFD, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CFD. You can calculate the concentration of Human CFD in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-108
94-104
92-105
Average (%)
102
99
99
1:4
Range (%)
92-102
94-105
86-96
Average (%)
98
101
93
1:8
Range (%)
95-109
91-107
96-103
Average (%)
102
99
99
1:16
Range (%)
100-109
90-103
93-103
Average (%)
103
96
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
100
EDTA plasma (n=5)
97-106
102
Cell culture media (n=5)
93-100
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.66
11.33
46.4
4.89
12.36
41.26
Standard deviation
0.28
0.57
1.92
0.24
0.6
2.12
C V (%)
6.01
5.03
4.14
4.91
4.85
5.14
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CFD concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CFD in samples. No significant cross-reactivity or interference between Human CFD and analogues was observed.
