The Human CFL1 (Cofilin-1 non-muscle) CLIA Kit is a powerful tool for accurately quantifying levels of cofilin-1 in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.Cofilin-1 is a key regulatory protein involved in actin dynamics, cell migration, and cytoskeletal organization. Dysregulation of cofilin-1 has been linked to various diseases, including cancer, neurodegenerative disorders, and inflammatory conditions.
As such, it serves as a valuable biomarker for studying these diseases and exploring potential therapeutic strategies.The Human CFL1 CLIA Kit from AssayGenie provides researchers with a reliable and accurate method for measuring cofilin-1 levels, enabling a deeper understanding of its role in health and disease. Trust in this kit for dependable results that drive groundbreaking discoveries in biomedical research.
Product Name:
Human CFL1 (Cofilin 1, Non-Muscle) CLIA Kit
SKU:
HUES00446
Target:
Human CFL1 (Cofilin 1, Non-Muscle)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CFL1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CFL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CFL1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CFL1. You can calculate the concentration of Human CFL1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-117
94-107
97-112
Average (%)
108
100
103
1:4
Range (%)
87-100
97-111
92-109
Average (%)
92
105
100
1:8
Range (%)
90-102
87-103
98-113
Average (%)
97
94
105
1:16
Range (%)
98-111
100-114
102-115
Average (%)
105
108
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
102
EDTA plasma (n=5)
98-112
105
Cell culture media (n=5)
88-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
102.63
261.99
710.76
92.74
281.2
723.65
Standard deviation
9.61
30.84
66.95
11.94
28.23
72.44
C V (%)
9.36
11.77
9.42
12.87
10.04
10.01
Sample type &Sample volume:
Tissue homogenates; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CFL1 concentrations in Tissue homogenates.
Specificity:
This kit recognizes Human CFL1 in samples. No significant cross-reactivity or interference between Human CFL1 and analogues was observed.
