Human CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) CLIA Kit
The Human CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) CLIA Kit is a specialized assay designed for the precise measurement of CFTR levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.CFTR is a vital protein involved in regulating ion transport across cell membranes, particularly in the respiratory and digestive systems. Mutations in the CFTR gene are linked to the development of cystic fibrosis, a genetic disorder that affects the lungs and digestive system.
The ability to accurately measure CFTR levels is essential for studying disease mechanisms, developing new therapies, and monitoring treatment effectiveness in cystic fibrosis patients.By using the Human CFTR CLIA Kit, researchers can gain valuable insights into the role of CFTR in health and disease, paving the way for improved diagnostic approaches and targeted treatment strategies for individuals affected by cystic fibrosis.
Product Name:
Human CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) CLIA Kit
SKU:
HUES00986
Target:
Human CFTR (Cystic Fibrosis Transmembrane Conductance Regulator)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CFTR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CFTR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CFTR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CFTR. You can calculate the concentration of Human CFTR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-99
86-98
101-117
Average (%)
92
92
109
1:4
Range (%)
90-102
89-103
87-99
Average (%)
96
94
93
1:8
Range (%)
89-101
84-97
101-116
Average (%)
95
91
109
1:16
Range (%)
91-105
98-112
89-102
Average (%)
99
106
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-111
102
EDTA plasma (n=5)
99-114
107
Cell culture media (n=5)
87-100
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.89
181.75
807.97
91.55
187.83
871.11
Standard deviation
9.57
13.16
75.79
11.79
22.22
73.52
C V (%)
9.88
7.24
9.38
12.88
11.83
8.44
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CFTR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CFTR in samples. No significant cross-reactivity or interference between Human CFTR and analogues was observed.
