Human Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) ELISA Kit
The Human CCL3L1 ELISA Kit is specifically designed for the precise quantification of CCL3L1 (C-C motif ligand 3-like protein 1) levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, perfect for various research applications.CCL3L1 is a key chemokine involved in immune responses and inflammation, playing a crucial role in conditions such as autoimmune diseases, infections, and inflammatory disorders.
Detecting and measuring CCL3L1 levels can provide valuable insights into disease pathology and potential therapeutic strategies.By using the Human CCL3L1 ELISA Kit, researchers can gain a better understanding of the role of CCL3L1 in health and disease, paving the way for the development of targeted treatment approaches and personalized medicine.
Product Name:
Human Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) ELISA Kit
Product Code:
HUDL00461
Size:
96 Assays
Target:
CCL3L1
Synonyms:
CCL3-L1, LD78, SCYA3L, SCYA3L1, MIP1AP, MIP1-Alpha-P, Small Inducible Cytokine A3-Like 1, Macrophage Inflammatory Protein 1-Alpha-P, Tonsillar Lymphocyte LD78 Beta
Detection Method:
Sandwich
Range:
78.125-5000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
