Human Chemokine C-X-C-Motif Ligand 16 (CXCL16) ELISA Kit
The Human C-X-C Motif Ligand 16 (CXCL16) ELISA Kit is a powerful tool for accurately measuring levels of CXCL16 in human biological samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers precise and reproducible results, making it an invaluable asset for a wide range of research applications.CXCL16 is a chemokine protein that plays a crucial role in immune responses and inflammation, functioning as a chemoattractant for immune cells.
Dysregulation of CXCL16 expression has been linked to various inflammatory conditions, autoimmune diseases, and cancer progression, highlighting its significance as a biomarker for understanding disease mechanisms and developing targeted therapies.Overall, the Human CXCL16 ELISA Kit provides researchers with a reliable and accurate method for studying the role of CXCL16 in health and disease, paving the way for new insights and potential treatment strategies.
Product Name:
Human Chemokine C-X-C-Motif Ligand 16 (CXCL16) ELISA Kit
Product Code:
HUDL00747
Size:
96 Assays
Target:
CXCL16
Synonyms:
SR-PSOX, CXCLG16, SRPSOX, Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein, Small-inducible cytokine B16
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
