Human Chemokine C-X-C-Motif Receptor 7 (CXCR7) ELISA Kit
The Human Chemokine C-X-C Motif Receptor 7 (CXCR7) ELISA Kit is a specialized assay kit designed to accurately measure the levels of CXCR7 in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and consistent results, making it a valuable tool for a wide range of research studies.CXCR7 is a chemokine receptor that plays a crucial role in various biological processes, including immune response, inflammation, and cancer progression.
By measuring CXCR7 levels, researchers can gain valuable insights into the role of this receptor in different diseases and potential therapeutic interventions.This ELISA kit offers a reliable and efficient method for quantifying CXCR7 levels, making it an essential tool for researchers studying chemokine signaling pathways, inflammation, and cancer biology. With its easy-to-use protocol and reproducible results, the Human CXCR7 ELISA Kit is an indispensable resource for advancing research in this field.
Product Name:
Human Chemokine C-X-C-Motif Receptor 7 (CXCR7) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
