Human Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) ELISA Kit
The Human Chemokine C-X3-C Motif Ligand 1 (CX3CL1) ELISA Kit is a reliable and accurate tool for detecting levels of CX3CL1 in human biological samples such as serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides researchers with dependable and reproducible results for a variety of research applications.CX3CL1, also known as fractalkine, is a unique chemokine involved in immune responses and inflammation. It plays a crucial role in mediating the adhesion and migration of immune cells, as well as regulating inflammatory processes.
Dysregulation of CX3CL1 has been implicated in various diseases, including autoimmune disorders, neurodegenerative diseases, and cancer.By accurately measuring CX3CL1 levels, researchers can gain valuable insights into the role of this chemokine in disease pathogenesis and progression. This ELISA kit is an essential tool for studying the function of CX3CL1 and exploring its potential as a therapeutic target in various conditions.
Product Name:
Human Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
