Human Chloride Channel Accessory 1 (CLCA1) ELISA Kit
The Human Chloride Channel Accessory 1 (CLCA1) ELISA Kit is a powerful tool for the precise measurement of CLCA1 levels in human samples such as serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable asset for various research applications.CLCA1 is a key regulator of chloride channels, playing a critical role in mucin synthesis, airway hydration, and mucociliary clearance.
Dysregulation of CLCA1 has been implicated in respiratory diseases such as asthma and chronic obstructive pulmonary disease (COPD), highlighting its importance as a potential diagnostic marker and therapeutic target.Discover the intricate role of CLCA1 in chloride channel function and respiratory health with the Human CLCA1 ELISA Kit. Trust in its precision and reliability to advance your research and contribute to the understanding and treatment of respiratory disorders.
Product Name:
Human Chloride Channel Accessory 1 (CLCA1) ELISA Kit
Product Code:
HUDL00582
Size:
96 Assays
Target:
CLCA1
Synonyms:
CaCC1, CLCRG1, Chloride Channel,Calcium Activated,Family Member 1, Calcium-activated chloride channel protein 1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
