The Human CK-17 (KRT17) Cytokeratin 17 CLIA Kit is specifically designed for the accurate detection of cytokeratin 17 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a wide range of research applications.Cytokeratin 17, also known as CK-17 or KRT17, is a type I cytokeratin protein that plays a crucial role in maintaining skin integrity and wound healing. Abnormal expression of CK-17 has been linked to various skin disorders, as well as certain types of cancer.
The Human CK-17 CLIA Kit provides researchers with a valuable tool for studying the role of cytokeratin 17 in disease progression and developing potential therapies.Overall, the Human CK-17 Cytokeratin 17 CLIA Kit is a valuable resource for researchers interested in investigating the function of cytokeratin 17 in health and disease. Its high sensitivity and specificity make it an ideal choice for accurate and reliable measurement of CK-17 levels in human samples.
Product Name:
Human CK-17/KRT17 (Cytokeratin 17) CLIA Kit
SKU:
HUES01104
Target:
Human CK-17/KRT17 (Cytokeratin 17)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CK17/KRT17. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CK17/KRT17 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CK17/KRT17, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CK17/KRT17. You can calculate the concentration of Human CK17/KRT17 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-112
89-103
94-109
Average (%)
104
96
101
1:4
Range (%)
101-114
91-106
87-99
Average (%)
107
98
92
1:8
Range (%)
94-105
98-112
99-109
Average (%)
100
103
104
1:16
Range (%)
102-114
93-111
98-112
Average (%)
108
101
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
91
EDTA plasma (n=5)
83-97
90
Cell culture media (n=5)
94-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
91.22
286.02
928.97
93.6
292.82
861.43
Standard deviation
10.75
32.78
102.0
8.26
25.53
57.72
C V (%)
11.78
11.46
10.98
8.82
8.72
6.7
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CK17/KRT17 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CK17/KRT17 in samples. No significant cross-reactivity or interference between Human CK17/KRT17 and analogues was observed.
