The Human CK-19 (KRT19) Cytokeratin 19 CLIA Kit is designed for the precise measurement of cytokeratin 19 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.Cytokeratin 19, also known as CK-19 or KRT19, is a key protein marker for various types of cancer, especially in the diagnosis and monitoring of epithelial tumors.
Its expression is indicative of the presence of epithelial cells and is commonly used in cancer research and clinical diagnostics.With this CLIA kit, researchers and healthcare professionals can easily quantify cytokeratin 19 levels, gaining valuable insights into disease progression, treatment response, and prognosis. This kit is an essential tool for advancing cancer research and improving patient care.
Product Name:
Human CK-19/KRT19 (Cytokeratin 19) CLIA Kit
SKU:
HUES01106
Target:
Human CK-19/KRT19 (Cytokeratin 19)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CK-19/KRT19. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CK-19/KRT19 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CK-19/KRT19, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CK-19/KRT19. You can calculate the concentration of Human CK-19/KRT19 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-114
84-96
83-95
Average (%)
106
91
90
1:4
Range (%)
91-102
85-97
99-115
Average (%)
97
91
106
1:8
Range (%)
89-99
90-103
103-118
Average (%)
94
97
110
1:16
Range (%)
100-115
95-108
102-114
Average (%)
107
101
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-107
102
EDTA plasma (n=5)
90-106
97
Cell culture media (n=5)
89-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.34
158.97
387.58
48.15
148.47
406.72
Standard deviation
5.81
12.22
37.83
4.93
15.74
41.24
C V (%)
12.02
7.69
9.76
10.24
10.6
10.14
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CK-19/KRT19 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CK-19/KRT19 in samples. No significant cross-reactivity or interference between Human CK-19/KRT19 and analogues was observed.
