The Human CK-20 (KRT20/Cytokeratin-20) CLIA Kit is specifically designed for the precise measurement of CK-20 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.CK-20, also known as Cytokeratin-20, is a cytoskeletal protein primarily found in normal and malignant epithelial cells. It is commonly used as a biomarker for detecting epithelial tumors, such as colorectal and urothelial carcinomas.
Monitoring CK-20 levels can aid in the diagnosis, prognosis, and treatment of these types of cancers.With its reliable performance and broad utility, the Human CK-20 CLIA Kit is an invaluable tool for researchers studying epithelial cell biology, cancer development, and personalized medicine. Trust in this kit to deliver precise measurements and valuable insights into CK-20 expression levels in diverse biological samples.
Product Name:
Human CK-20/KRT20 (Cytokeratin 20) CLIA Kit
SKU:
HUES01107
Target:
Human CK-20/KRT20 (Cytokeratin 20)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CK20/KRT20. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CK20/KRT20 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CK20/KRT20, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CK20/KRT20. You can calculate the concentration of Human CK20/KRT20 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-95
93-110
100-118
Average (%)
90
100
108
1:4
Range (%)
85-101
103-119
85-98
Average (%)
92
109
91
1:8
Range (%)
89-104
86-100
97-111
Average (%)
95
91
102
1:16
Range (%)
88-100
96-111
101-117
Average (%)
93
102
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-113
105
EDTA plasma (n=5)
96-108
103
Cell culture media (n=5)
93-104
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.01
302.59
881.38
106.86
317.05
943.21
Standard deviation
8.48
24.99
69.28
10.94
22.48
79.89
C V (%)
8.65
8.26
7.86
10.24
7.09
8.47
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CK20/KRT20 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CK20/KRT20 in samples. No significant cross-reactivity or interference between Human CK20/KRT20 and analogues was observed.
