The Human CKM (Creatine Kinase Muscle) CLIA Kit is a cutting-edge assay designed for the precise measurement of CKM levels in human samples such as serum, plasma, and cell culture supernatants. With superior sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable tool for a variety of research applications.CKM is an important enzyme found predominantly in muscle tissue, playing a key role in energy metabolism and muscle function.
Abnormal CKM levels can be indicative of muscle damage or disease, making it a valuable biomarker for studying conditions such as muscular dystrophy, heart disease, and sports-related injuries.With its advanced technology and user-friendly format, the Human CKM CLIA Kit from AssayGenie is the perfect solution for researchers looking to accurately measure CKM levels and gain valuable insights into muscle health and function. Order yours today and revolutionize your research!
Product Name:
Human CKM (Creatine Kinase, Muscle) CLIA Kit
SKU:
HUES00812
Target:
Human CKM (Creatine Kinase, Muscle)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CKM. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CKM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CKM, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CKM. You can calculate the concentration of Human CKM in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-116
96-110
100-117
Average (%)
107
104
108
1:4
Range (%)
94-106
92-106
93-107
Average (%)
101
99
98
1:8
Range (%)
88-99
89-101
88-99
Average (%)
94
96
93
1:16
Range (%)
99-110
97-113
97-114
Average (%)
104
104
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-105
96
EDTA plasma (n=5)
90-104
97
Cell culture media (n=5)
88-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.01
2.9
7.04
0.95
2.79
6.59
Standard deviation
0.1
0.29
0.52
0.08
0.2
0.52
C V (%)
9.9
10.0
7.39
8.42
7.17
7.89
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CKM concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CKM in samples. No significant cross-reactivity or interference between Human CKM and analogues was observed.
