The Human Claudin-4 (CLDN4) ELISA Kit is specifically developed for the precise measurement of Claudin-4 levels in human biological samples including serum, plasma, and cell culture supernatants. This ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.Claudin-4 is a key protein involved in epithelial barrier function and plays a critical role in regulating cell-cell adhesion and permeability. Dysregulation of Claudin-4 expression has been linked to various diseases such as cancer, inflammatory bowel disease, and viral infections.
Therefore, the measurement of Claudin-4 levels can provide valuable insights into disease pathogenesis and progression, as well as potential therapeutic interventions.With its reliable performance and user-friendly protocol, the Human Claudin-4 ELISA Kit is an indispensable tool for researchers studying epithelial biology, disease mechanisms, and drug development. Order yours today to accelerate your research and advance scientific discoveries in the field.
Product Name:
Human Claudin 4 (CLDN4) ELISA Kit
Product Code:
HUDL00588
Size:
96 Assays
Target:
CLDN4
Synonyms:
CPE-R, CPER, CPETR, CPETR1, WBSCR8, hCPE-R, Clostridium Perfringens Enterotoxin Receptor 1, Williams-Beuren Syndrome Chromosomal Region 8 Protein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
