Human CLCF1 (Cardiotrophin Like Cytokine Factor 1) CLIA Kit
The Human CLCF1 (Cardiotrophin-like cytokine factor 1) CLIA Kit is a highly accurate and sensitive assay designed for the detection of CLCF1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it an ideal tool for various research applications.CLCF1 is a critical cytokine factor involved in various physiological processes, including immune regulation, inflammation, and cell growth.
Dysregulation of CLCF1 has been implicated in a variety of diseases, making it a valuable biomarker for studying these conditions and potentially discovering novel therapeutic interventions.By using the Human CLCF1 CLIA Kit, researchers can gain valuable insights into the role of CLCF1 in disease pathogenesis and progression, paving the way for innovative approaches to diagnosis and treatment.
Product Name:
Human CLCF1 (Cardiotrophin Like Cytokine Factor 1) CLIA Kit
SKU:
HUES00431
Target:
Human CLCF1 (Cardiotrophin Like Cytokine Factor 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CLCF1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CLCF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CLCF1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CLCF1. You can calculate the concentration of Human CLCF1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
92-107
96-112
Average (%)
99
98
103
1:4
Range (%)
91-104
103-118
100-118
Average (%)
98
109
108
1:8
Range (%)
84-96
94-106
95-110
Average (%)
91
100
103
1:16
Range (%)
91-104
86-98
87-100
Average (%)
97
92
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
93
EDTA plasma (n=5)
94-110
102
Cell culture media (n=5)
92-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
39.47
92.73
303.04
37.21
100.65
324.43
Standard deviation
3.88
8.65
22.97
3.36
8.83
21.54
C V (%)
9.83
9.33
7.58
9.03
8.77
6.64
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CLCF1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CLCF1 in samples. No significant cross-reactivity or interference between Human CLCF1 and analogues was observed.
