The Human CLDN11 (Claudin-11) CLIA Kit is a highly reliable assay designed for the precise quantification of CLDN11 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and reproducible results for a variety of research applications.CLDN11, also known as Claudin-11, is a critical protein involved in cell-cell adhesion and the formation of tight junctions in various tissues, particularly in the central nervous system. Dysregulation of CLDN11 has been implicated in conditions such as multiple sclerosis and other neuroinflammatory disorders, making it a valuable biomarker for understanding disease mechanisms and developing potential therapeutic interventions.
With its superior performance and versatility, the Human CLDN11 CLIA Kit is an invaluable tool for researchers seeking to explore the role of Claudin-11 in health and disease, providing precise measurements for meaningful insights and discoveries in the field of molecular biology and beyond.
Product Name:
Human CLDN11 (Claudin 11) CLIA Kit
SKU:
HUES00514
Target:
Human CLDN11 (Claudin 11)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CLDN11. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CLDN11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CLDN11, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CLDN11. You can calculate the concentration of Human CLDN11 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-116
99-114
101-120
Average (%)
107
105
109
1:4
Range (%)
99-112
101-117
101-118
Average (%)
105
107
109
1:8
Range (%)
90-101
90-104
99-114
Average (%)
96
95
106
1:16
Range (%)
95-110
98-111
85-98
Average (%)
103
104
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-114
107
EDTA plasma (n=5)
89-103
94
Cell culture media (n=5)
87-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
106.09
299.69
954.31
104.16
329.59
933.23
Standard deviation
11.32
27.33
103.92
12.78
38.33
92.95
C V (%)
10.67
9.12
10.89
12.27
11.63
9.96
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CLDN11 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CLDN11 in samples. No significant cross-reactivity or interference between Human CLDN11 and analogues was observed.
