The Human CLDN4 (Claudin-4) CLIA Kit is specifically designed for the quantitative measurement of CLDN4 levels in human biological samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results for a variety of research applications.Claudin-4 is a key protein in the tight junctions of epithelial cells, playing a crucial role in maintaining the integrity and barrier function of tissues. Dysregulation of CLDN4 has been associated with various diseases, including cancer, inflammatory bowel disease, and hepatitis C infection.
Therefore, studying CLDN4 levels can provide valuable insights into the pathogenesis of these conditions and potentially identify new therapeutic targets.Overall, the Human CLDN4 CLIA Kit from Assay Genie offers researchers a powerful tool to investigate the role of Claudin-4 in health and disease, ultimately advancing our understanding of cellular biology and opening up new avenues for drug development.
Product Name:
Human CLDN4 (Claudin 4) CLIA Kit
SKU:
HUES00502
Target:
Human CLDN4 (Claudin 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CLDN4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CLDN4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CLDN4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CLDN4. You can calculate the concentration of Human CLDN4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-104
85-97
89-100
Average (%)
95
90
95
1:4
Range (%)
91-103
83-99
100-118
Average (%)
96
90
108
1:8
Range (%)
98-111
93-105
89-102
Average (%)
105
100
97
1:16
Range (%)
88-99
95-107
101-117
Average (%)
94
102
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-105
100
EDTA plasma (n=5)
98-110
104
Cell culture media (n=5)
85-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.91
257.13
941.04
102.07
258.0
1013.35
Standard deviation
11.98
26.69
89.59
8.52
25.39
89.99
C V (%)
11.42
10.38
9.52
8.35
9.84
8.88
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CLDN4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CLDN4 in samples. No significant cross-reactivity or interference between Human CLDN4 and analogues was observed.
