The Human CLDN9 (Claudin-9) CLIA Kit is specifically designed for the accurate measurement of CLDN9 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it an excellent choice for various research applications.Claudin-9 is a crucial protein that belongs to the claudin family and plays a significant role in tight junction formation, cell adhesion, and barrier function in epithelial cells.
Dysregulation of CLDN9 has been associated with various diseases such as cancer, inflammatory bowel disease, and neurological disorders, highlighting its importance as a potential biomarker for disease diagnosis and therapeutic development.Overall, the Human CLDN9 CLIA Kit offers researchers a valuable tool for studying the role of CLDN9 in disease pathogenesis and developing targeted therapies for conditions where CLDN9 dysregulation is implicated.
Product Name:
Human CLDN9 (Claudin 9) CLIA Kit
SKU:
HUES00498
Target:
Human CLDN9 (Claudin 9)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CLDN9. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CLDN9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CLDN9, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CLDN9. You can calculate the concentration of Human CLDN9 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-105
96-112
99-117
Average (%)
97
102
106
1:4
Range (%)
87-98
95-108
92-107
Average (%)
93
102
100
1:8
Range (%)
87-99
86-98
91-107
Average (%)
93
92
98
1:16
Range (%)
88-102
98-111
99-113
Average (%)
95
104
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-104
99
EDTA plasma (n=5)
87-98
93
Cell culture media (n=5)
89-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.38
104.94
446.34
53.69
111.81
407.67
Standard deviation
7.0
9.65
44.05
4.88
8.02
39.67
C V (%)
12.87
9.2
9.87
9.09
7.17
9.73
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CLDN9 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CLDN9 in samples. No significant cross-reactivity or interference between Human CLDN9 and analogues was observed.
