Human CLEC4E (C-Type Lectin Domain Family 4 Member E) ELISA Kit
The Human CLEC4E (C-Type Lectin Domain Family 4, Member E) ELISA Kit is a dependable tool for the precise measurement of CLEC4E levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers consistent and accurate results, making it well-suited for a variety of research applications.CLEC4E is a key protein known for its involvement in immune responses, particularly in the recognition and binding of pathogens.
Its role in modulating immune functions makes it a valuable target for studying infectious diseases, autoimmune disorders, and inflammatory conditions. By utilizing this ELISA kit, researchers can gain valuable insights into the functions and mechanisms of CLEC4E, aiding in the development of new therapeutic strategies.
Product Name:
Human CLEC4E (C-Type Lectin Domain Family 4 Member E) ELISA Kit
SKU:
HUES01990
Target:
Human CLEC4E (C-Type Lectin Domain Family 4 Member E)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CLEC4E. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CLEC4E and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CLEC4E, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CLEC4E. You can calculate the concentration of Human CLEC4E in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-110
88-102
97-110
Average (%)
103
94
104
1:4
Range (%)
93-108
82-95
87-101
Average (%)
99
89
94
1:8
Range (%)
89-106
80-93
85-95
Average (%)
97
86
90
1:16
Range (%)
91-104
84-98
86-100
Average (%)
98
91
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-104
98
EDTA plasma (n=5)
90-102
96
Cell culture media (n=5)
93-105
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
244.41
742.23
2143.6
233.43
733.88
2131.05
Standard deviation
12.34
30.21
99.25
15.69
33.39
78.64
C V (%)
5.05
4.07
4.63
6.72
4.55
3.69
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CLEC4E concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CLEC4E in samples. No significant cross-reactivity or interference between Human CLEC4E and analogues was observed.
