Human Cluster Of Differentiation 30 Ligand (CD30L) ELISA Kit
The Human Cluster of Differentiation 30 Ligand (CD30L) ELISA Kit is a reliable and sensitive tool designed for the precise detection of CD30L levels in human serum, plasma, and cell culture supernatants. This kit offers high specificity and accuracy, ensuring consistent and trustworthy results for a variety of research applications.CD30L is a key protein involved in the immune response and plays a critical role in regulating T cell activation and proliferation.
Dysregulation of CD30L expression has been associated with various immune-related disorders, making it a valuable biomarker for studying immune system function and potential therapeutic interventions.With its advanced capabilities and ease of use, the Human CD30L ELISA Kit is a valuable asset for researchers in the fields of immunology, oncology, and autoimmune diseases, providing valuable insights into the role of CD30L in health and disease.
Product Name:
Human Cluster Of Differentiation 30 Ligand (CD30L) ELISA Kit
Product Code:
HUDL00489
Size:
96 Assays
Target:
CD30L
Synonyms:
CD153, TNFSF8, CD30-L, Tumor Necrosis Factor Ligand Superfamily Member 8
Detection Method:
Sandwich
Range:
78.125-5000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-102
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
