Human CNTNAP2 (Contactin Associated Protein Like Protein 2) CLIA Kit
The Human CNTNAP2 (Contactin-associated protein-like protein 2) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of CNTNAP2 levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.CNTNAP2 is a protein that plays a crucial role in neuronal development and function, particularly in synapse formation and maintenance. Mutations in the CNTNAP2 gene have been associated with neurodevelopmental disorders such as autism spectrum disorder and intellectual disabilities, making it an important biomarker for understanding and potentially treating these conditions.
With its high sensitivity and specificity, the Human CNTNAP2 CLIA Kit is a valuable tool for researchers studying neurological disorders and seeking to uncover the mechanisms underlying these conditions. Its accurate detection capabilities make it an essential component for advancing our knowledge of CNTNAP2 biology and its implications for human health.
Product Name:
Human CNTNAP2 (Contactin Associated Protein Like Protein 2) CLIA Kit
SKU:
HUES00247
Target:
Human CNTNAP2 (Contactin Associated Protein Like Protein 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CNTNAP2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CNTNAP2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CNTNAP2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CNTNAP2. You can calculate the concentration of Human CNTNAP2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
89-105
87-99
Average (%)
94
96
94
1:4
Range (%)
84-97
87-100
84-100
Average (%)
91
94
91
1:8
Range (%)
86-101
92-104
90-103
Average (%)
93
99
97
1:16
Range (%)
96-113
97-109
92-104
Average (%)
103
102
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-102
93
EDTA plasma (n=5)
86-99
93
Cell culture media (n=5)
87-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.27
265.47
942.51
105.93
241.62
871.35
Standard deviation
10.05
23.92
78.04
10.08
28.0
71.54
C V (%)
10.33
9.01
8.28
9.52
11.59
8.21
Sample type &Sample volume:
Tissue homogenates; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CNTNAP2 concentrations in Tissue homogenates.
Specificity:
This kit recognizes Human CNTNAP2 in samples. No significant cross-reactivity or interference between Human CNTNAP2 and analogues was observed.
