The Human CNX (Calnexin) ELISA Kit is specifically designed for the precise measurement of calnexin levels in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and consistent results, making it an excellent tool for various research applications.Calnexin is a vital protein involved in protein folding and quality control within the endoplasmic reticulum.
Dysregulation of calnexin has been linked to various diseases, including cancer, neurodegenerative disorders, and autoimmune diseases. Therefore, the Human CNX ELISA Kit serves as a valuable tool for studying these conditions and potentially identifying new therapeutic targets.
Product Name:
Human CNX (Calnexin) ELISA Kit
SKU:
HUES01789
Target:
Human CNX (Calnexin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/ml
Detection range:
0.63-40 ng/ml
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CNX. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CNX and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CNX, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CNX. You can calculate the concentration of Human CNX in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
98-114
94-109
Average (%)
100
104
102
1:4
Range (%)
87-100
88-101
85-96
Average (%)
93
93
91
1:8
Range (%)
90-107
85-97
85-95
Average (%)
97
92
90
1:16
Range (%)
93-108
86-99
81-97
Average (%)
99
93
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
98
EDTA plasma (n=5)
92-104
98
Cell culture media (n=5)
94-106
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.82
5.34
15.98
1.83
5.09
17.28
Standard deviation
0.12
0.3
0.56
0.12
0.21
0.79
C V (%)
6.59
5.62
3.5
6.56
4.13
4.57
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CNX concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CNX in samples. No significant cross-reactivity or interference between Human CNX and analogues was observed.
