The Human Coenzyme Q10 Homolog B (CoQ10B) ELISA Kit is a reliable and accurate tool for detecting levels of CoQ10B in human serum, plasma, and cell culture supernatants. This ELISA kit offers high sensitivity and specificity, ensuring trustworthy and consistent results for a variety of research applications.Coenzyme Q10 Homolog B is a vital enzyme involved in cellular energy production and antioxidant defense. It plays a critical role in maintaining cellular health and protecting against oxidative stress, making it a valuable biomarker for studying conditions related to mitochondrial dysfunction, cardiovascular diseases, and neurodegenerative disorders.
By using the Human Coenzyme Q10 Homolog B ELISA Kit, researchers can gain valuable insights into cellular energy metabolism, oxidative stress, and potential therapeutic targets for a range of health conditions. This kit is user-friendly, efficient, and ensures accurate quantification of CoQ10B levels, providing researchers with valuable data for their studies.
Product Name:
Human Coenzyme Q10 Homolog B (COQ10B) ELISA Kit
Product Code:
HUDL00638
Size:
96 Assays
Target:
COQ10B
Synonyms:
Coenzyme Q-binding protein COQ10 homolog B, mitochondrial
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-102
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
