The Human COL10 (Collagen Type X) ELISA Kit is a specialized assay designed for the quantitative determination of Collagen Type X levels in various human samples. Collagen Type X is a vital component of the extracellular matrix, particularly involved in the development and maintenance of bone and cartilage tissues. This specific collagen type is known for its role in endochondral ossification, the process by which cartilage turns into bone during skeletal development. Accurate measurement of Collagen Type X is crucial for studying skeletal development, cartilage formation, and bone growth.
Changes in the levels of Collagen Type X can serve as biomarkers for various bone disorders, growth plate pathologies, and skeletal diseases. This ELISA kit provides exceptional sensitivity and specificity, ensuring precise and reproducible results. With robust performance and user-friendly protocols, this kit is ideal for research and clinical applications. Trust Assay Genie's COL10 ELISA Kit for accurate and reliable quantification of this key matrix protein in your investigations.
Product Name:
Human COL10 (Collagen Type X) ELISA Kit
SKU:
AEES00086
Target:
Human COL10 (Collagen Type Ⅹ)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human COL10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human COL10. You can calculate the concentration of Human COL10 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
83-93
95-103
83-96
Average (%)
90
99
90
1:4
Range (%)
83-97
90-100
94-104
Average (%)
91
94
99
1:8
Range (%)
98-111
95-106
94-108
Average (%)
103
102
101
1:16
Range (%)
96-107
85-97
89-95
Average (%)
104
91
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-102
94
EDTA plasma (n=5)
88-103
95
Cell culture media (n=5)
88-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
86.83
232.59
850.8
96.39
242.27
836.8
Standard deviation
4.63
10.93
37.95
5.5
11.7
34.98
C V (%)
5.33
4.7
4.46
5.71
4.83
4.18
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human COL10 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COL10 in samples. No significant cross-reactivity or interference between Human COL10 and analogues was observed.
