The Human Collagen Type V Alpha 2 (COL5A2) ELISA Kit is a reliable and accurate tool for the quantitative detection of COL5A2 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.COL5A2 is a key component of collagen type V, which plays a crucial role in maintaining the structural integrity and mechanical properties of tissues such as skin, tendons, and blood vessels.
Abnormalities in COL5A2 expression have been linked to various connective tissue disorders, making it an important biomarker for studying these conditions and developing potential therapeutic strategies.With its user-friendly protocol and high-performance capabilities, the Human Collagen Type V Alpha 2 (COL5A2) ELISA Kit is an indispensable tool for researchers and clinicians seeking to better understand the role of COL5A2 in health and disease.
Product Name:
Human Collagen Type V Alpha 2 (COL5a2) ELISA Kit
Product Code:
HUDL00627
Size:
96 Assays
Target:
COL5a2
Synonyms:
COL5-A2, Collagen Alpha-2(V)chain, AB Collagen
Detection Method:
Sandwich
Range:
78.12-5000ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
