Description
Human COLEC11 (Collectin Sub-Family Member 11) ELISA Kit
The Human COLEC11 (Collectin Sub-Family Member 11) ELISA Kit is a powerful tool crafted for the precise measurement of Collectin Sub-Family Member 11 levels in various human samples. COLEC11, a member of the collectin family, is involved in innate immunity and plays a crucial role in pathogen recognition and host defense mechanisms.
This ELISA kit enables researchers to accurately quantify COLEC11 levels, shedding light on its implications in inflammatory responses, immune regulation, and disease pathogenesis. Accurate quantification of COLEC11 is essential for unraveling its functions in immune responses, infectious diseases, and inflammatory disorders. By offering exceptional sensitivity and specificity, this ELISA kit ensures reliable and reproducible results. Its robust performance and user-friendly design make it an ideal choice for both laboratory research and clinical applications. Rely on Assay Genie's COLEC11 ELISA Kit for trustworthy and precise measurement of this significant biomarker in your studies.
| Product Name: | Human COLEC11 (Collectin Sub-Family Member 11) ELISA Kit |
| Product Code: | AEES00087 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Human |
| Detection Range: | 78.13-5000 pg/mL |
| Sensitivity: | 46.88 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Human COLEC11 in samples. No significant cross-reactivity or interference between Human COLEC11 and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Human COLEC11 concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human COLEC11. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COLEC11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COLEC11, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human COLEC11. You can calculate the concentration of Human COLEC11 in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
