Human Colony Stimulating Factor 2 Receptor Beta (CSF2Rb) ELISA Kit
The Human Colony Stimulating Factor 2 Receptor Beta (CSF2RB) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of CSF2RB levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a wide range of research applications.CSF2RB is a critical receptor involved in the regulation of immune responses and hematopoiesis. It plays a key role in mediating the effects of colony-stimulating factors on immune cell development and function. Dysregulation of CSF2RB signaling has been implicated in various diseases, including autoimmune disorders and hematologic malignancies, making it a valuable target for therapeutic interventions.
By using the Human CSF2RB ELISA Kit, researchers can accurately quantify CSF2RB levels in biological samples, allowing for a better understanding of its role in disease pathogenesis and the development of novel treatment strategies. This kit provides a valuable tool for investigating the mechanisms underlying immune system regulation and hematopoietic disorders.
Product Name:
Human Colony Stimulating Factor 2 Receptor Beta (CSF2Rb) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
