Human Colony Stimulating Factor Receptor, Granulocyte (GCSFR) ELISA Kit
The Human Colony Stimulating Factor Receptor (Granulocyte) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of G-CSFR levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for various research applications related to the immune system and hematopoiesis.G-CSFR is a critical receptor involved in the regulation of granulocyte production and function. Dysregulation of G-CSFR signaling has been linked to various hematological disorders, including leukemia and myeloproliferative neoplasms.
Therefore, the detection of G-CSFR levels using this ELISA kit can provide valuable insights into the pathogenesis of these diseases and aid in the development of targeted therapeutic strategies.Overall, the Human Colony Stimulating Factor Receptor (Granulocyte) ELISA Kit is a powerful tool for researchers studying immune function, hematopoiesis, and hematological disorders, offering high sensitivity and specificity for accurate quantification of G-CSFR levels in biological samples.
Product Name:
Human Colony Stimulating Factor Receptor, Granulocyte (GCSFR) ELISA Kit
Product Code:
HUDL01162
Size:
96 Assays
Target:
GCSFR
Synonyms:
CD114, G-CSFR, CSF3R, CSF3-R, GCSF-R
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
