Human Complement Factor H Related Protein 3 (CFHR3) ELISA Kit
The Human Complement Factor H Related Protein 3 (CFHR3) ELISA Kit is specially designed for the accurate and precise measurement of CFHR3 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures reliable and reproducible results, making it a valuable tool for various research applications.CFHR3 is a member of the complement factor H-related protein family, playing a role in the regulation of the complement system and modulation of immune responses.
Dysregulation of CFHR3 has been linked to various autoimmune diseases, inflammatory disorders, and renal conditions, highlighting its importance as a potential biomarker for disease diagnosis and therapeutic development.Overall, the Human CFHR3 ELISA Kit offers researchers a reliable and efficient method for studying the role of CFHR3 in health and disease, advancing our understanding of the complement system and its implications for human health.
Product Name:
Human Complement Factor H Related Protein 3 (CFHR3) ELISA Kit
Product Code:
HUDL00544
Size:
96 Assays
Target:
CFHR3
Synonyms:
CFHL3, DOWN16, FHR-3, FHR3, HLF4, H factor-like protein 3
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
