The Human Cortisol ELISA Kit is designed for the quantitative detection of cortisol levels in various biological samples.
Cortisol is a vital glucocorticoid hormone produced by the adrenal glands, pivotal in managing the body's stress response, maintaining metabolic functions, and controlling inflammation. It is essential in a wide array of bodily activities, including blood sugar regulation, fat, protein, and carbohydrate metabolism, and immune response. Accurate measurement of cortisol is crucial for understanding its involvement in stress-related disorders, endocrine diseases, metabolic syndrome, and for developing targeted therapeutic strategies. Assay Genie's Human Cortisol ELISA Kit offers outstanding sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under stringent quality control standards, this kit provides robust performance and is easy to use, making it an excellent choice for both research and clinical applications. Trust Assay Genie's Human Cortisol ELISA Kit for accurate and dependable quantification of this critical biomarker in your studies.
Product Name:
Human Cortisol ELISA Kit
SKU:
AEES00016
Target:
Human Cortisol
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
2.92 ng/mL
Detection range:
6.25-400 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Cortisol. During the reaction, Cortisol in the sample or standard competes with a fixed amount of Cortisol on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Cortisol. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cortisol in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
80-90
88-102
85-107
Average (%)
85
93
94
1:4
Range (%)
81-96
87-95
80-101
Average (%)
88
90
92
1:8
Range (%)
84-102
82-89
83-95
Average (%)
95
86
86
1:16
Range (%)
87-104
89-104
84-99
Average (%)
93
99
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
81-93
85
EDTA plasma (n=5)
86-96
89
Cell culture media (n=5)
85-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.33
100.32
200.52
51.27
99.84
202.18
Standard deviation
4.42
8.08
14.9
4.59
7.02
14.46
C V (%)
8.78
8.05
7.43
8.96
7.03
7.15
Sample type &Sample volume:
serum, plasma, urine; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Cortisol concentrations in serum, plasma, urine.
Specificity:
This kit recognizes Cortisol in samples. No significant cross-reactivity or interference between Cortisol and analogues was observed.
