Description
Product Name: | Human Coxsackievirus A16 (CV-A16) IgM ELISA Kit |
SKU: | HDES0080 |
Size: | 96T |
Target: | CV-A16-IgM |
Detection Method: | Capture ELISA |
Reactivity: | Human |
Result Type: | Qualitative: Sensitivity 98.3%, Specificity 99.3% |
Sample Type: | Serum, Plasma |
Detection Wavelength: | 450/630nm |
Storage: | The majority of kits have a shelf-life of 12 months from the production date at 2-8℃ |
This ELISA kit uses Capture-ELISA as the method to detect the CA16-IgM in the sample. The micro-plate is pre-coated with Mouse-anti-human IgM (μ chain). CA16-IgM in samples will be captured after adding the samples to wells. After washing completely, add CA16 antigen conjugated Horseradish Peroxidase (HRP), and it will react with the “anti-μ chain CA16 IgM” compound to form “anti-μ chain- CA16 IgM-HRP conjugated CA16 antigen”. Wash and remove the free components. Add the TMB substrate to initiate color developing reaction. The presence of CA16-IgM antibody can be determined according to the OD value after colorimetric assay with the Micro-plate Reader.