The Human CR (Calretinin) CLIA Kit is a highly reliable assay kit designed for the precise measurement of calretinin levels in human samples. Calretinin is a calcium-binding protein that plays a vital role in various biological processes, including cell differentiation and nerve cell function.This kit offers high sensitivity and specificity, providing accurate and reproducible results for research applications in fields such as neuroscience, oncology, and developmental biology.
Calretinin levels can serve as a valuable biomarker for identifying various diseases and conditions, making this kit an essential tool for understanding the role of calretinin in health and disease.Overall, the Human CR (Calretinin) CLIA Kit is a powerful tool for researchers seeking to investigate the functions and implications of calretinin in human biology, offering precise measurements and reliable data to support groundbreaking discoveries.
Product Name:
Human CR (Calretinin) CLIA Kit
SKU:
HUES00415
Target:
Human CR (Calretinin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CR. You can calculate the concentration of Human CR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-111
87-100
94-104
Average (%)
104
92
99
1:4
Range (%)
88-104
103-120
90-103
Average (%)
96
110
98
1:8
Range (%)
96-109
95-109
102-117
Average (%)
103
102
108
1:16
Range (%)
99-114
89-104
95-109
Average (%)
105
95
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-112
106
EDTA plasma (n=5)
93-106
99
Cell culture media (n=5)
90-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
221.34
397.21
1543.95
213.97
404.61
1679.2
Standard deviation
25.96
29.67
94.64
27.82
47.1
181.02
C V (%)
11.73
7.47
6.13
13.0
11.64
10.78
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CR in samples. No significant cross-reactivity or interference between Human CR and analogues was observed.
