The Human CR1 (Complement Receptor Type 1) CLIA Kit is specifically designed for the accurate quantification of CR1 levels in human samples including serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring robust and reproducible results for various research applications.CR1, also known as CD35, is a key player in the immune system, specifically in the regulation of the complement system. It is involved in the clearance of immune complexes and pathogens, as well as in controlling inflammation.
Dysregulation of CR1 has been linked to various autoimmune diseases, infections, and inflammatory conditions, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.Overall, the Human CR1 CLIA Kit offers researchers a reliable tool for investigating the role of CR1 in health and disease, with the potential to contribute to the development of novel diagnostic and therapeutic strategies.
Product Name:
Human CR1 (Complement Receptor type 1) CLIA Kit
SKU:
HUES01153
Target:
Human CR1 (Complement Receptor type 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CR1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CR1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CR1. You can calculate the concentration of Human CR1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-109
101-115
88-104
Average (%)
103
107
95
1:4
Range (%)
99-117
91-106
98-114
Average (%)
107
97
105
1:8
Range (%)
100-114
89-102
96-111
Average (%)
108
96
103
1:16
Range (%)
98-115
100-117
95-110
Average (%)
106
107
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-111
101
EDTA plasma (n=5)
86-99
93
Cell culture media (n=5)
92-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.53
43.66
198.87
26.76
47.9
211.98
Standard deviation
2.75
4.76
14.18
3.13
3.43
21.03
C V (%)
10.77
10.9
7.13
11.7
7.16
9.92
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CR1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CR1 in samples. No significant cross-reactivity or interference between Human CR1 and analogues was observed.
