Human CRLF1 (Cytokine Receptor Like Factor 1) CLIA Kit
The Human CRLF1 (Cytokine Receptor-Like Factor 1) CLIA Kit is a cutting-edge assay designed for the precise measurement of CRLF1 levels in human samples such as serum, plasma, and cell culture supernatants. This advanced kit offers unparalleled sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.CRLF1 is a key protein involved in various biological processes, including immune response and inflammatory pathways.
Dysregulation of CRLF1 has been linked to several diseases, making it a valuable biomarker for studying and developing treatments for conditions such as inflammatory disorders and autoimmune diseases.With its reliable performance and easy-to-use format, the Human CRLF1 CLIA Kit is an essential tool for researchers looking to explore the role of CRLF1 in health and disease. Unlock new insights and accelerate your research with this state-of-the-art assay kit.
Product Name:
Human CRLF1 (Cytokine Receptor Like Factor 1) CLIA Kit
SKU:
HUES00257
Target:
Human CRLF1 (Cytokine Receptor Like Factor 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CRLF1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CRLF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CRLF1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CRLF1. You can calculate the concentration of Human CRLF1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-101
91-105
101-116
Average (%)
95
99
108
1:4
Range (%)
85-97
87-98
101-115
Average (%)
90
92
108
1:8
Range (%)
99-114
99-116
85-100
Average (%)
105
107
91
1:16
Range (%)
96-108
101-116
89-104
Average (%)
102
108
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
91
EDTA plasma (n=5)
92-105
99
Cell culture media (n=5)
97-111
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.09
230.64
674.62
103.18
215.23
707.3
Standard deviation
10.54
18.04
44.79
8.78
19.41
67.48
C V (%)
10.97
7.82
6.64
8.51
9.02
9.54
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CRLF1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CRLF1 in samples. No significant cross-reactivity or interference between Human CRLF1 and analogues was observed.
