The Human CSF2R (Colony Stimulating Factor 2 Receptor) Beta CLIA Kit is specifically designed for the accurate detection of CSF2R Beta levels in human samples including serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring consistent and reliable results for a variety of research applications.CSF2R Beta is a key receptor involved in the immune response, specifically in the differentiation and proliferation of myeloid cells.
Dysregulation of this receptor has been linked to various inflammatory and autoimmune diseases, making it a valuable biomarker for research in these areas.The Human CSF2R Beta CLIA Kit from AssayGenie provides researchers with a powerful tool for studying the role of CSF2R Beta in health and disease, and for developing potential therapeutic interventions. Trust in the accuracy and precision of this kit for your research needs.
Human CSF2R beta (Colony Stimulating Factor 2 Receptor Beta)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CSF2Rβ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CSF2Rβ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CSF2Rβ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CSF2Rβ. You can calculate the concentration of Human CSF2Rβ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-109
85-97
92-104
Average (%)
102
91
98
1:4
Range (%)
88-100
96-113
97-110
Average (%)
95
104
104
1:8
Range (%)
93-104
95-106
89-101
Average (%)
98
101
94
1:16
Range (%)
93-106
90-104
97-111
Average (%)
100
98
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-115
106
EDTA plasma (n=5)
94-108
101
Cell culture media (n=5)
93-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
93.63
211.51
811.64
87.71
195.8
837.74
Standard deviation
11.33
20.37
63.31
11.18
18.15
58.39
C V (%)
12.1
9.63
7.8
12.75
9.27
6.97
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CSF2Rβ concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CSF2Rβ in samples. No significant cross-reactivity or interference between Human CSF2Rβ and analogues was observed.
