The Human CT-1 (Cardiotrophin-1) ELISA Kit is a powerful tool for the precise measurement of cardiotrophin-1 levels in human biological samples, including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable resource for various research applications.Cardiotrophin-1 is a key regulator of cardiac hypertrophy and has been implicated in multiple cardiovascular diseases, making it a valuable biomarker for studying heart-related conditions and exploring potential treatment strategies.
By utilizing this ELISA kit, researchers can gain valuable insights into the role of cardiotrophin-1 in disease progression and development, ultimately advancing our understanding of cardiovascular health.
Product Name:
Human CT-1 (Cardiotrophin 1) ELISA Kit
SKU:
HUES01812
Target:
Human CT-1 (Cardiotrophin 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CT1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CT1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CT1. You can calculate the concentration of Human CT1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-102
91-106
94-104
Average (%)
96
98
99
1:4
Range (%)
90-102
80-93
86-101
Average (%)
96
87
93
1:8
Range (%)
90-104
85-99
83-93
Average (%)
96
91
89
1:16
Range (%)
93-104
81-95
86-101
Average (%)
98
87
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-105
97
EDTA plasma (n=5)
94-109
100
Cell culture media (n=5)
86-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
53.62
85.92
421.46
58.39
82.88
452.35
Standard deviation
3.72
3.57
22.59
3.7
3.57
23.07
C V (%)
6.94
4.16
5.36
6.34
4.31
5.1
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CT1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CT1 in samples. No significant cross-reactivity or interference between Human CT1 and analogues was observed.
