Human CTACK (Cutaneous T-cell Attracting Chemokine) CLIA Kit
The Human CTACK (Cutaneous T-cell Attracting Chemokine) CLIA Kit is a highly sensitive assay designed for the accurate quantification of CTACK levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional specificity and reproducibility, making it an invaluable tool for researchers in various fields.CTACK is a chemokine known for attracting T-cells to the skin, playing a crucial role in the immune response and inflammatory processes. Dysregulation of CTACK expression has been implicated in various skin disorders, autoimmune diseases, and inflammatory conditions, highlighting its significance as a biomarker for studying these diseases and exploring potential therapeutic interventions.
By utilizing the Human CTACK CLIA Kit, researchers can gain valuable insights into the role of CTACK in immune responses, inflammation, and skin diseases, ultimately advancing our understanding of these complex processes and paving the way for novel treatment strategies.
Product Name:
Human CTACK (Cutaneous T-cell Attracting Chemokine) CLIA Kit
SKU:
HUES00032
Target:
Human CTACK (Cutaneous T-cell Attracting Chemokine)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CTACK. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CTACK and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CTACK, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CTACK. You can calculate the concentration of Human CTACK in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-119
100-117
85-100
Average (%)
109
108
91
1:4
Range (%)
93-105
91-105
101-119
Average (%)
99
96
110
1:8
Range (%)
91-105
87-101
98-109
Average (%)
98
92
104
1:16
Range (%)
85-99
98-116
90-105
Average (%)
91
106
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-113
107
EDTA plasma (n=5)
94-107
101
Cell culture media (n=5)
99-114
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
36.42
69.49
380.71
39.32
72.52
365.96
Standard deviation
3.35
5.69
23.76
3.22
6.63
35.13
C V (%)
9.2
8.19
6.24
8.19
9.14
9.6
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CTACK concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CTACK in samples. No significant cross-reactivity or interference between Human CTACK and analogues was observed.
