Human CTGF (Connective Tissue Growth Factor) CLIA Kit
The Human CTGF (Connective Tissue Growth Factor) CLIA Kit is a specialized assay designed for the accurate measurement of CTGF levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.CTGF is a key regulatory protein that plays a critical role in the growth and maintenance of connective tissues. It is involved in processes such as cell adhesion, migration, proliferation, and tissue repair. Dysregulation of CTGF has been implicated in various diseases such as fibrosis, cancer, and arthritis, highlighting its potential as a biomarker for studying these conditions and exploring therapeutic interventions.
With its advanced technology and user-friendly format, the Human CTGF CLIA Kit from Assay Genie provides researchers with a valuable tool for investigating the role of CTGF in health and disease, ultimately advancing our understanding of connective tissue biology and paving the way for potential diagnostic and treatment strategies.
Product Name:
Human CTGF (Connective Tissue Growth Factor) CLIA Kit
SKU:
HUES00507
Target:
Human CTGF (Connective Tissue Growth Factor)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CTGF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CTGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CTGF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CTGF. You can calculate the concentration of Human CTGF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-107
94-108
96-109
Average (%)
101
99
104
1:4
Range (%)
92-109
96-112
91-103
Average (%)
99
103
96
1:8
Range (%)
88-102
97-111
99-113
Average (%)
95
104
105
1:16
Range (%)
95-110
94-108
86-98
Average (%)
102
100
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-109
102
EDTA plasma (n=5)
91-105
97
Cell culture media (n=5)
98-110
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.99
107.29
487.85
52.58
96.98
520.0
Standard deviation
4.52
8.13
44.3
5.99
8.84
44.67
C V (%)
8.69
7.58
9.08
11.39
9.12
8.59
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CTGF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CTGF in samples. No significant cross-reactivity or interference between Human CTGF and analogues was observed.
