Human cTnT/TNNT2 (Troponin T Type 2, Cardiac) ELISA Kit
The Human cTnT (Troponin T Type 2) Cardiac ELISA Kit is specially designed for the precise measurement of cTnT levels in human samples such as serum, plasma, and cell culture supernatants. This ELISA kit offers superior sensitivity and specificity, ensuring accurate and consistent results for various research purposes.cTnT is a key cardiac marker indicative of myocardial injury and is commonly used in the diagnosis and monitoring of acute coronary syndromes. Elevated cTnT levels are associated with heart conditions such as myocardial infarction and heart failure, underscoring its importance in clinical research and patient care.
With this advanced ELISA kit, researchers can analyze cTnT levels with confidence, enabling them to better understand cardiac health and develop more effective treatments for cardiovascular diseases. Trust in the Human cTnT Cardiac ELISA Kit for reliable and insightful results in your cardiac research endeavors.
Product Name:
Human cTnT/TNNT2 (Troponin T Type 2, Cardiac) ELISA Kit
SKU:
HUES01807
Target:
Human cTnT/TNNT2 (Troponin T Type 2, Cardiac)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human cTnT/TNNT2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human cTnT/TNNT2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human cTnT/TNNT2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human cTnT/TNNT2. You can calculate the concentration of Human cTnT/TNNT2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-109
87-100
94-107
Average (%)
99
93
100
1:4
Range (%)
89-104
83-96
83-94
Average (%)
95
89
89
1:8
Range (%)
91-104
83-96
86-100
Average (%)
96
89
91
1:16
Range (%)
92-105
87-101
84-98
Average (%)
98
93
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
94
EDTA plasma (n=5)
93-104
98
Cell culture media (n=5)
91-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
45.94
100.24
399.56
47.33
99.68
413.65
Standard deviation
2.79
4.31
18.3
2.49
4.39
17.79
C V (%)
6.07
4.3
4.58
5.26
4.4
4.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human cTnT/TNNT2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human cTnT/TNNT2 in samples. No significant cross-reactivity or interference between Human cTnT/TNNT2 and analogues was observed.
