The Human CTSD (Cathepsin D) CLIA Kit is specially designed for the precise measurement of Cathepsin D levels in human serum samples. Cathepsin D is a lysosomal protease involved in various physiological processes, including protein degradation, autophagy, and cell death. Elevated levels of Cathepsin D have been linked to several diseases, such as cancer, Alzheimer's, and cardiovascular disorders.
This CLIA kit offers exceptional sensitivity and specificity, ensuring accurate and reliable results for research and diagnostic purposes. It is suitable for a wide range of applications in the field of biomarker discovery, drug development, and disease monitoring. Order now and unlock the potential of Cathepsin D as a valuable biomarker in your research studies.
Product Name:
Human CTSD (Cathepsin D) CLIA Kit
SKU:
HUES00440
Target:
Human CTSD (Cathepsin D)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CTSD. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CTSD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CTSD, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CTSD. You can calculate the concentration of Human CTSD in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-105
99-113
90-104
Average (%)
100
107
98
1:4
Range (%)
97-113
87-101
89-99
Average (%)
104
93
94
1:8
Range (%)
90-106
91-106
92-105
Average (%)
97
99
98
1:16
Range (%)
88-102
100-117
102-118
Average (%)
95
108
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-117
107
EDTA plasma (n=5)
100-113
107
Cell culture media (n=5)
97-109
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.8
135.43
338.17
49.78
136.96
323.89
Standard deviation
4.88
12.92
36.22
6.05
13.37
31.81
C V (%)
10.21
9.54
10.71
12.15
9.76
9.82
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CTSD concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CTSD in samples. No significant cross-reactivity or interference between Human CTSD and analogues was observed.
