The Human CTSK (Cathepsin K) CLIA Kit is specifically designed for the precise and accurate measurement of Cathepsin K levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results that are well-suited for various research purposes.Cathepsin K is an important enzyme involved in bone resorption and remodeling, playing a critical role in diseases such as osteoporosis and osteoarthritis.
By analyzing Cathepsin K levels, researchers can gain insights into the mechanisms of bone turnover and potentially identify new therapeutic targets for these conditions. Overall, the Human CTSK CLIA Kit provides researchers with a valuable tool for studying Cathepsin K and its implications in bone health and related diseases, ultimately leading to advancements in understanding and treating these conditions.
Product Name:
Human CTSK (Cathepsin K) CLIA Kit
SKU:
HUES00441
Target:
Human CTSK (Cathepsin K)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CTSK. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CTSK and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CTSK, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CTSK. You can calculate the concentration of Human CTSK in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-116
89-101
100-117
Average (%)
107
94
108
1:4
Range (%)
89-105
100-117
104-116
Average (%)
97
107
110
1:8
Range (%)
97-110
86-100
94-105
Average (%)
103
93
99
1:16
Range (%)
95-107
104-117
89-102
Average (%)
101
110
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
94
EDTA plasma (n=5)
87-98
92
Cell culture media (n=5)
91-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
107.5
247.33
853.64
115.29
243.36
802.14
Standard deviation
13.44
18.1
56.6
14.72
23.65
56.55
C V (%)
12.5
7.32
6.63
12.77
9.72
7.05
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CTSK concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CTSK in samples. No significant cross-reactivity or interference between Human CTSK and analogues was observed.
