The Human CTSS (Cathepsin S) ELISA Kit is a reliable tool for the precise measurement of Cathepsin S levels in human samples, including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers consistent and accurate results, making it an invaluable resource for various research applications.Cathepsin S is a key enzyme involved in the degradation of proteins, particularly in the immune system and inflammatory processes.
Dysregulated Cathepsin S levels have been linked to various diseases, including autoimmune disorders, atherosclerosis, and cancer. Therefore, this ELISA kit provides researchers with valuable insights into the role of Cathepsin S in disease pathology, paving the way for potential therapeutic interventions.
Product Name:
Human CTSS (Cathepsin S) ELISA Kit
SKU:
HUES03432
Target:
Human CTSS (Cathepsin S)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CTSS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CTSS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CTSS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CTSS. You can calculate the concentration of Human CTSS in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
95-109
85-99
Average (%)
103
103
92
1:4
Range (%)
93-104
86-97
88-103
Average (%)
98
92
94
1:8
Range (%)
87-99
83-96
81-94
Average (%)
93
88
88
1:16
Range (%)
92-106
83-95
89-103
Average (%)
99
90
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
99
EDTA plasma (n=5)
88-104
95
Cell culture media (n=5)
85-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.48
1.33
4.21
0.5
1.37
4.41
Standard deviation
0.03
0.06
0.2
0.03
0.06
0.13
C V (%)
6.25
4.51
4.75
6.0
4.38
2.95
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CTSS concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CTSS in samples. No significant cross-reactivity or interference between Human CTSS and analogues was observed.
