The Human CTSV (Cathepsin V) CLIA Kit is a reliable and accurate tool for detecting cathepsin V levels in human biological samples. This kit is highly sensitive and specific, providing researchers with trustworthy and reproducible results for various research applications.Cathepsin V, an important protease enzyme, is involved in various physiological processes, including immune response regulation, tissue remodeling, and antigen presentation. Abnormal cathepsin V levels have been linked to various diseases, such as cancer, autoimmune disorders, and inflammatory conditions, making it a valuable biomarker for studying and developing potential treatments for these diseases.
Overall, the Human CTSV CLIA Kit is a valuable tool for researchers seeking to investigate the role of cathepsin V in human health and disease, offering accurate and reliable assays for measuring cathepsin V levels in biological samples.
Product Name:
Human CTSV (Cathepsin V) CLIA Kit
SKU:
HUES00019
Target:
Human CTSV (Cathepsin V)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CTSV. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CTSV and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CTSV, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CTSV. You can calculate the concentration of Human CTSV in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-116
97-111
91-106
Average (%)
110
104
97
1:4
Range (%)
98-115
86-100
94-105
Average (%)
107
93
100
1:8
Range (%)
95-107
92-109
99-113
Average (%)
100
100
106
1:16
Range (%)
90-107
102-117
99-115
Average (%)
97
110
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-114
105
EDTA plasma (n=5)
96-112
102
Cell culture media (n=5)
97-111
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.25
109.2
369.91
46.37
100.73
384.17
Standard deviation
6.16
11.5
29.04
3.83
7.5
27.31
C V (%)
12.26
10.53
7.85
8.26
7.45
7.11
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CTSV concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CTSV in samples. No significant cross-reactivity or interference between Human CTSV and analogues was observed.
