Human CTX-II (Cross Linked C-telopeptide of Type II Collagen) ELISA Kit
The Human CTX-II (Cross-linked C-telopeptide of Type II Collagen) ELISA Kit is specifically designed for the accurate measurement of CTX-II levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results that are perfect for a variety of research applications.CTX-II is a key biomarker associated with the degradation of type II collagen in cartilage, making it a valuable marker for studying conditions such as osteoarthritis and rheumatoid arthritis.
By measuring CTX-II levels, researchers can gain insights into cartilage degradation, disease progression, and potential therapeutic interventions.Overall, the Human CTX-II ELISA Kit provides researchers with a reliable tool for studying cartilage degradation and monitoring disease progression, offering valuable insights for the development of new treatments for conditions related to type II collagen degradation.
Product Name:
Human CTX-II (Cross Linked C-telopeptide of Type II Collagen) ELISA Kit
SKU:
HUES01971
Target:
Human CTX-II (Cross Linked C-telopeptide of Type II Collagen)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CTXⅡ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CTXⅡ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CTXⅡ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CTXⅡ. You can calculate the concentration of Human CTXⅡ in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-112
95-107
90-103
Average (%)
103
100
96
1:4
Range (%)
98-114
80-94
95-107
Average (%)
105
86
100
1:8
Range (%)
96-110
87-100
92-108
Average (%)
102
93
99
1:16
Range (%)
99-112
79-90
98-109
Average (%)
104
86
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-103
98
EDTA plasma (n=5)
88-98
93
Cell culture media (n=5)
85-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.1
4.6
0.5
1.0
4.2
Standard deviation
0.03
0.05
0.14
0.03
0.06
0.2
C V (%)
6
4.55
3.04
6.0
6.0
4.76
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CTXâ…¡ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CTXâ…¡ in samples. No significant cross-reactivity or interference between Human CTXâ…¡ and analogues was observed.
