SynonymsLithostathine-1-beta,Pancreatic stone protein 2,PSP-2,PSPS2,REG1B,REG-1-beta,Regenerating islet-derived protein 1-beta,Regenerating protein I beta,REGL
ReactivityHuman
Sample TypeTissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
SKU:
HUDL00738
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q86UP6
Sensitivity:
0.114ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
Lithostathine-1-beta,Pancreatic stone protein 2,PSP-2,PSPS2,REG1B,REG-1-beta,Regenerating islet-derived protein 1-beta,Regenerating protein I beta,REGL
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
Human CUB And Zona Pellucida Like Domains Protein 1 (CUZD1) ELISA Kit
The Human CUZD1 (CUB and Zona Pellucida-like Domains Protein 1) ELISA Kit is a highly reliable and sensitive assay designed for the accurate measurement of CUZD1 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional specificity and precision, ensuring consistent and trustworthy results for a variety of research purposes.CUZD1 is a protein that contains CUB and zona pellucida-like domains, and is involved in several biological processes, including cell adhesion and receptor binding.
It is known to play a role in various disease states, such as cancer and autoimmune disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Human CUZD1 ELISA Kit provides researchers with a powerful tool to investigate the role of CUZD1 in health and disease, ultimately advancing our understanding of its biological functions and potential clinical applications.
Product Name:
Human CUB And Zona Pellucida Like Domains Protein 1 (CUZD1) ELISA Kit
Product Code:
HUDL00738
Size:
96 Assays
Target:
CUZD1
Synonyms:
Lithostathine-1-beta,Pancreatic stone protein 2,PSP-2,PSPS2,REG1B,REG-1-beta,Regenerating islet-derived protein 1-beta,Regenerating protein I beta,REGL
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
