The Human CX26 (Connexin-26) CLIA Kit is specifically designed for the precise measurement of CX26 levels in human samples including serum, plasma, and cell culture supernatants. This advanced kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.CX26, a member of the connexin protein family, plays a critical role in cell-cell communication through gap junction channels. Dysregulation of CX26 has been implicated in various disorders such as hearing loss, skin diseases, and certain cancers, making it a valuable biomarker for understanding disease mechanisms and developing targeted therapies.
With its high performance and versatility, the Human CX26 CLIA Kit is an essential tool for researchers investigating the role of CX26 in disease pathogenesis and exploring its potential as a therapeutic target. Unlock new insights and advance your research with this innovative assay kit.
Product Name:
Human CX26 (Connexin 26) CLIA Kit
SKU:
HUES00508
Target:
Human CX26 (Connexin 26)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CX26. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CX26 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CX26, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CX26. You can calculate the concentration of Human CX26 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
83-96
94-109
Average (%)
101
90
101
1:4
Range (%)
92-103
94-105
100-117
Average (%)
98
99
107
1:8
Range (%)
97-109
87-98
85-98
Average (%)
103
92
91
1:16
Range (%)
102-117
96-112
94-111
Average (%)
109
103
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
97
EDTA plasma (n=5)
97-112
103
Cell culture media (n=5)
92-110
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
216.37
548.24
1748.65
195.94
506.64
1901.47
Standard deviation
20.01
54.28
122.76
22.32
45.39
159.34
C V (%)
9.25
9.9
7.02
11.39
8.96
8.38
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CX26 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CX26 in samples. No significant cross-reactivity or interference between Human CX26 and analogues was observed.
