The Human CX40 (Connexin-40) CLIA Kit is specifically designed for the precise measurement of CX40 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results for a variety of research purposes.CX40, also known as Connexin-40, is a key protein involved in gap junction channels and intercellular communication. It plays a crucial role in various physiological processes, including cardiac conduction and vascular function.
Dysregulation of CX40 has been linked to conditions such as cardiovascular diseases, arrhythmias, and vascular disorders, highlighting its importance as a biomarker for understanding these pathologies and exploring potential therapeutic interventions.Overall, the Human CX40 CLIA Kit provides researchers with a valuable tool for investigating the role of CX40 in health and disease, offering reliable and reproducible data to advance our understanding of this critical protein.
Product Name:
Human CX40 (Connexin 40) CLIA Kit
SKU:
HUES00941
Target:
Human CX40 (Connexin 40)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CX40. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CX40 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CX40, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CX40. You can calculate the concentration of Human CX40 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-100
92-107
95-111
Average (%)
92
97
102
1:4
Range (%)
100-113
92-104
102-113
Average (%)
108
98
108
1:8
Range (%)
102-120
97-110
93-106
Average (%)
109
104
99
1:16
Range (%)
98-111
88-102
91-105
Average (%)
104
94
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
93
EDTA plasma (n=5)
96-112
102
Cell culture media (n=5)
92-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
216.55
500.7
1700.59
207.77
496.04
1629.53
Standard deviation
23.69
53.12
143.19
24.6
38.39
106.25
C V (%)
10.94
10.61
8.42
11.84
7.74
6.52
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CX40 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CX40 in samples. No significant cross-reactivity or interference between Human CX40 and analogues was observed.
