The Human CX43 (Connexin-43) CLIA Kit is specifically designed for the quantitative measurement of Connexin-43 levels in human biological samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results, making it suitable for a variety of research applications.Connexin-43 is a key protein involved in cell-to-cell communication through gap junction channels. Dysregulation of Connexin-43 has been implicated in various diseases including cardiovascular disorders, neurodegenerative diseases, and certain cancers.
Therefore, this kit serves as a valuable tool for studying the role of Connexin-43 in these conditions and developing potential therapeutic interventions.Overall, the Human CX43 CLIA Kit from Assay Genie offers researchers a robust and efficient solution for studying the role of Connexin-43 in health and disease, providing valuable insights for future advancements in biomedical research.
Product Name:
Human CX43 (Connexin 43) CLIA Kit
SKU:
HUES00942
Target:
Human CX43 (Connexin 43)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CX43. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CX43 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CX43, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CX43. You can calculate the concentration of Human CX43 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-116
86-100
99-114
Average (%)
109
92
107
1:4
Range (%)
89-101
92-104
89-101
Average (%)
94
99
95
1:8
Range (%)
85-96
98-115
97-109
Average (%)
91
106
104
1:16
Range (%)
87-99
103-117
87-102
Average (%)
94
109
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-112
105
EDTA plasma (n=5)
89-101
94
Cell culture media (n=5)
96-110
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
92.92
177.23
905.47
85.28
186.46
899.82
Standard deviation
9.52
19.81
67.46
10.49
13.41
54.89
C V (%)
10.25
11.18
7.45
12.3
7.19
6.1
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CX43 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CX43 in samples. No significant cross-reactivity or interference between Human CX43 and analogues was observed.
