Human CXCL16 (Chemokine C-X-C-Motif Ligand 16) CLIA Kit
The Human CXCL16 (Chemokine C-X-C motif ligand 16) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of CXCL16 levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a wide range of research applications.CXCL16 is a chemokine that plays a critical role in immune responses and inflammatory processes. It is involved in regulating the migration and activation of immune cells, as well as promoting the recruitment of immune cells to sites of inflammation.
Dysregulation of CXCL16 has been linked to various diseases, including autoimmune disorders, inflammatory conditions, and cancer.By accurately measuring CXCL16 levels, researchers can better understand the role of this chemokine in health and disease, leading to potential insights for developing novel therapies and diagnostic tools. Upgrade your research with the Human CXCL16 CLIA Kit today.
Product Name:
Human CXCL16 (Chemokine C-X-C-Motif Ligand 16) CLIA Kit
SKU:
HUES00052
Target:
Human CXCL16 (Chemokine C-X-C-Motif Ligand 16)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CXCL16. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CXCL16 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CXCL16, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CXCL16. You can calculate the concentration of Human CXCL16 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-103
95-109
93-105
Average (%)
95
102
99
1:4
Range (%)
88-100
99-116
96-111
Average (%)
95
107
103
1:8
Range (%)
89-105
101-116
90-105
Average (%)
96
106
98
1:16
Range (%)
92-106
100-113
103-118
Average (%)
100
107
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-117
107
EDTA plasma (n=5)
86-98
93
Cell culture media (n=5)
99-113
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
103.1
241.1
860.18
95.01
241.48
790.84
Standard deviation
11.45
17.72
81.72
12.08
22.51
90.63
C V (%)
11.11
7.35
9.5
12.71
9.32
11.46
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CXCL16 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CXCL16 in samples. No significant cross-reactivity or interference between Human CXCL16 and analogues was observed.
